Data associated with "Amyloid formation of alternatively spliced variants of α-synuclein"

Source data-Aggregation kinetics

Figure 2A and S2: Aggregation kinetics of SNCA and spliced variants under high agitation conditions

Figure 3A and S5: Aggregation kinetics of SNCA and spliced variants under low agitation conditions

Figure 3B and S6: Aggregation kinetics of SNCAΔ5 and SNCAΔ3Δ5 at low protein concentrations

Figure 4A and S12: Cross-seeding reactions of soluble SNCA with spliced variant fibrils at a seed concentration of 5 mol%

Figure 5A and B and S14: Co-mixing reactions of soluble SNCA with alternatively spliced variant monomer

Figure S10: Comparison of aggregation kinetics of SNCA and spliced variants at pH 5

Figure S11: Self-seeding reactions of SNCA and spliced variants at seed concentration of 5 mol%

Figure S13: Cross-seeding reactions of soluble SNCA with spliced variant fibrils at a seed concentration of 10 mol%

Figures and Tables

Figure 1B: SDS-PAGE analysis of purified recombinant SNCA and spliced variants.

Figure 1C: CD spectra of soluble SNCA and spliced variants.

Figure 2B: CD spectra taken post-aggregation of SNCA and spliced variant fibrils.

Figure 2C and Figure S3: Representative TEM images of SNCA and spliced variant fibrils formed under high agitation conditions.

Figure 3C and Figure S7: Representative TEM images of SNCA and spliced variant fibrils formed under low agitation conditions.

Figure 4A: SDS-PAGE analysis of soluble and insoluble fractions post-aggregation.

Figure 4B: CD spectra of insoluble fraction of SNCA seeded by SNCAΔ5.

Figure 4C and D: Representative TEM image of self-seeded SNCA and SNCA cross-seeded with 5% SNCAΔ5 fibrils

Figure 5A and B: SDS-PAGE analysis of soluble (S) and insoluble (I) fractions after ultracentrifugation post-aggregation of SNCA alone, SNCA co-mixed with 25 and 5 µM SNCAΔ3, SNCAΔ5, and SNCAΔ3Δ5, respectively.

Figure 5C: TEM images of SNCA co-mixed with either SNCAΔ5 or SNCAΔ3Δ5

Table S1. MS analysis of limited PK-digestion of fibrils

Figure S1. LC traces of purified SNCA and spliced variants

Figure S4. SDS-PAGE analysis of PK-digestion of fibrils

Figure S8. Histogram reporting fibril half pitches of SNCAΔ5 and SNCAΔ3Δ5

Figure S9. CD spectra of SNCA and spliced variants post-aggregation under low agitation conditions