Data associated with "Defining essential charged residues in fibril formation of a lysosomal derived N-terminal alpha-synuclein truncation

<p dir="ltr"><b>Source data</b>:</p><ul><li>Figure 1d-g: Activity assays for CtsB, CtsL, CtsD and AEP</li><li>Figure 2a: ThT aggregation kinetic data for Ac1-140 and 66-140</li><li>Figure 2c and S6: Raman data on Ac1-140 and 66-140 fibrils</li><li>Figure 3a: 2D ¹³C-¹³C ssNMR data for 66-140 fibrils</li><li>Figure 3b: Secondary chemical shift data for 66-140</li><li>Figure 4b and S14: ThT aggregation kinetic data for 66-140 and single ala-mutants</li><li>Figure 5a and S16: Raman data 66-140 and single ala-mutant</li><li>Figure 5b: Raman difference data subtracting 66-140 from single ala-mutants</li><li>Figure 5c: ThT aggregation kinetic data of soluble 66-140 seeded with single ala-mutant fibrils</li><li>Figure 6a: ThT aggregation kinetic data of single ala-mutants seeded with 66-140 fibrils</li><li>Figure 6c and S22: Raman data of single-Ala mutants seeded with 66–140 fibrils</li><li>Figure 7a: ThT aggregation kinetic data of charge switch mutants seeded with 66-140 fibrils</li><li>Figure 7c and S24: Raman data of E83K/K97E seeded with 66–140 fibrils</li><li>Figure 8b: ThT aggregation kinetic data for Ac1-140</li><li>Figure 8c: ThT aggregation kinetic data AcK80A</li><li>Figure 8d: ThT aggregation kinetic data AcK96A</li><li>Figure 8e: ThT aggregation kinetic data AcK97A</li><li>Figure 8g: ThT aggregation kinetic data of soluble Ac1-140 seeded with single-Ala full-length mutants</li><li>Figure S3: Data for a salt-dependent aggregation of 66–140</li><li>Figure S4a: ThT aggregation kinetic data for self-seeding 66‒140</li><li>Figure S7a: 2D carbon-carbon cross-polarization data</li><li>Figure S7b: Data for 1D slices along the ¹³C dimension at indicated positions in 2D carbon-carbon spectrum</li><li>Figure S8a: 2D NCACX data on 66‒140 fibrils</li><li>Figure S8b: Data for 1D slices along the ¹³C dimension at indicated positions in 2D NCACX spectrum</li><li>Figure S9a: 2D NCOCX data on 66‒140 fibrils</li><li>Figure S9b: Data for 1D slices along the ¹³C dimension at indicated positions in 2D NCOCX spectrum</li><li>Figure S10a and S10b: 3D NCACX data on labeled 66‒140 fibrils showing representative 2D planes at constant ¹⁵N frequencies</li><li>Figure S10c and S10d: Data for 1D slices along the ¹³C dimension at indicated positions in 3D NCaCX spectrum</li><li>Figure S11a and S11b: 3D NCOCX spectra on labeled 66‒140 fibrils showing representative 2D planes at constant ¹⁵N frequencies</li><li>Figure S11c and S11d: Data for 1D slices along the ¹³C dimension at indicated positions in 3D NCaCX spectrum</li><li>Figure S12a and S12b: 3D NCACX data on labeled 66‒140 fibrils showing representative 2D planes at constant ¹³Ca frequencies</li><li>Figure S12c and S12d: Data for 1D slices along the ¹³C dimension at indicated positions in 3D NCaCX spectrum</li><li>Figure S13a: Data for 1D ¹³C on labeled 66-140 fibrils</li><li>Figure S13b: Data for 1D ¹⁵N on labeled 66-140 fibrils</li><li>Figure S13c: Data for frequency-selective REDOR experiments</li><li>Figure S16: Raman data of single-Ala mutants</li><li>Figure S17: ThT aggregation kinetic data on cross-seeding of soluble 66‒140</li><li>Figure S18: ThT aggregation kinetic data on E104A/E105A seeded with 66‒140 fibrils</li><li>Figure S19a and S19b:ThT aggregation kinetic data on K96A/K97A and cross-seeding by 66‒140 fibrils</li><li>Figure S20:ThT aggregation kinetic data of soluble single-Ala mutants seeded with 66‒140 fibrils</li><li>Figure S25b: 66-140 molecular dynamic simulation data on radial distribution functions for distance of K80, K96 and K97 to E83 in 66-140</li><li>Figure S25d: 66-140 molecular dynamic simulation data for salt bridges occurring over 15-ns</li><li>Figure S26: ThT aggregation kinetic data of Ac1-140 and single Ala-mutants</li><li>Figure S28: Raman data for Ac1–140 and single Ala-mutants</li></ul><p dir="ltr"><b>Figures and Tables</b></p><p dir="ltr"><br></p><p dir="ltr">Figure 2b and S5a: TEM image of 66-140 fibrils</p><p dir="ltr">Figure 2b and S5b: AFM image of 66-140 fibrils</p><p dir="ltr">Figure 3c: Structural model of the 66‒140 fibril</p><p dir="ltr">Figure 4c and S15: TEM images of K80A, E83A, K96A, K97A, D98A and K102A fibrils</p><p dir="ltr">Figure 4c and S15: AFM images of K80A, E83A, K96A, K97A, D98A and K102A fibrils</p><p dir="ltr">Figure 6B and S21: TEM images of K80A, E83A, K96A, K97A, D98A and K102A mutants seeded with 66-140 fibrils</p><p dir="ltr">Figure 7B and S23: TEM image of E83K/K97E seeded with 66‒140 fibrils</p><p dir="ltr">Figure 8F and S27: TEM images of Ac1‒140 and single Ala-mutants</p><p dir="ltr">Supplementary Figure 1a: SDS-PAGE gel showing CtsB degradation of soluble syn</p><p dir="ltr">Supplementary Figure 1b: SDS-PAGE gel showing CtsL degradation of soluble syn</p><p dir="ltr">Supplementary Figure 1c: SDS-PAGE gel showing AEP degradation of soluble syn</p><p dir="ltr">Supplementary Figure 1d: SDS-PAGE gel showing CtsD degradation of soluble syn</p><p dir="ltr">Supplementary Figure 3: TEM images 66–140 fibrils taken post aggregation in 0.4 and 0.8 M NaCl</p><p dir="ltr">Supplementary Figure 4B: TEM images of 66–140 seeded with 66-140 fibrils</p><p dir="ltr">Supplementary Figure 25: Data for 66–140 model structure by molecular dynamics simulations</p><p dir="ltr">Supplementary Table 6: PK digestion of 66‒140 fibrils</p><p dir="ltr">Supplementary Table 7: Reported _tlag and _t½ for 66–140 and single-Ala aggregation kinetic data</p><p dir="ltr">Supplementary Table 8: PK digestion of single-Ala mutant fibrils</p><p><br></p><p><br></p><p><br></p><p><br></p>