DOCUMENT

Full unedited blots for Figure 4D.pdf (753.41 kB)

DOCUMENT

Full unedited blots for Figure 5A.pdf (652.27 kB)

DATASET

Fig 6B_RWD_Ctrl vs NR.xlsx (13.29 kB)

DATASET

Fig 6C_WC_Ctrl vs NR.xlsx (13.32 kB)

DATASET

Fig 6D_RWD_S3KDNR.xlsx (12.3 kB)

DATASET

Fig 6E_WC_S3KDNR.xlsx (12.24 kB)

DATASET

Fig 6F_RWD_S3OE.xlsx (13.53 kB)

DATASET

Fig 6G_WC_S3OE.xlsx (13.47 kB)

1/0

8 files

Primary data associated with the manuscript entitled "Nicotinamide Riboside Augments Human Macrophage Migration via SIRT3 Mediated Prostaglandin E2 Signaling".

dataset

posted on 2024-02-16, 17:56 authored by Jing WuJing Wu, Maximilian Bley, Russell Steans, Allison Meadows, Rebecca Huffstutler, Rong Tian, Julian Griffin, Michael SackMichael Sack

NAD⁺-boosting via nicotinamide riboside (NR) confers anti-inflammatory effects. However, underlying mechanisms and therapeutic potential remain incompletely defined. Here we showed NR increased the expression of CC-chemokine receptor 7 (CCR7) in human M1 macrophage by flow cytometric analysis of cell surface receptors. Consequently, chemokine ligand 19 (CCL19, ligand for CCR7) - induced macrophage migration was enhanced following NR administration. Metabolomics analysis revealed Prostaglandin E2 (PGE2) was increased by NR in human monocytes and in human serum following in vivo NR supplementation. Furthermore, NR-mediated upregulation of macrophage migration through CCL19-CCR7 was dependent on PGE2 synthesis. We also demonstrated that NR upregulates PGE2 synthesis through SIRT3-dependent post-transcriptional regulation of Cyclooxygenase 2 (COX-2). The NR-SIRT3-Migration axis was further validated by the scratch-test model where NR and Sirt3 promoted more robust migration across a uniformly disrupted macrophage monolayer. Thus, NR-mediated metabolic regulation on macrophage migration and wound healing may have therapeutic potential for the topical management of chronic wound healing.