Data for "<strong>Condensin dysfunction and peri-centromere expansion create a reproductive isolating barrier in mice</strong>"

<p>Microscopy and Western blot images analyzed in the Figures and Extended Data Figures in <strong>El Yakoubi and Akera,</strong> "<strong>Condensin dysfunction and peri-centromere expansion create a reproductive isolating barrier in mice</strong>"</p> <p><br></p> <p><strong>Abstract:</strong></p> <p>Reproductive isolation occurs when the genomes of two populations accumulate genetic incompatibilities that prevent inter-breeding. Cell biological understanding of such hybrid incompatibility is limited, especially for hybrid female sterility. Here we find that species divergence in condensin regulation and centromere organization between two mouse species, <em>Mus musculus</em> <em>domesticus</em> and <em>Mus spretus</em>, drives chromosome de-condensation and mis-segregation in their F1 hybrid oocytes, reducing female fertility. The chromosome condensation defects in hybrid oocytes were especially prominent at <em>Mus musculus</em> <em>domesticus </em>centromeres due to their highly abundant peri-centromeric major satellite DNA, leading to species-specific chromosome mis-segregation. This study provides the first mechanistic insights into hybrid incompatibility in female meiosis and demonstrates that the combination of condensin mis-regulation and peri-centromere expansion can establish a reproductive isolating barrier in mammals.</p>